The phases of Mitosis are: Interphase, Prophase, Metaphase, Anaphase, and Telephase. During Interphase, cell grows and the DNA is replicated. The DNA must be replicated before anything else can take place. In Prophase, the sister chromatids pair up. The chromatids are together. During Metaphase, the chromosomes line up at the center. Since this is Mitosis they line up horizontally. If this were Meiosis they chromosomes would line up vertically. During Anaphase the sister chromatids are pulled apart. During telephase you have two new cells.