*Put 1 cm of solvent in a 50 mL graduated cylinder
*Cut a apiece of filter paper and draw a pencil line, 1.5 cm above the point
*Use a coin and roll over the spinach leaf 8 to 10 times
*Place the chromatography paper in the cylinder, the point should barely be in the solvent
*Remove when the solvent is about 1 cm from the top of the paper
*mark the bottom of each pigment
I observed that the green from the spinach leave moved up the paper once the solvent was in it. Each pigment were different colors. They went from a lime greenish, to a darker green, to a dark yellow, to a yellow, and finally to a yellow/orangish.
Chromatography helps to separate and identify pigments. In the lab the solvent moved up the paper through capillary action. As the solvent moves up the paper, it takes the substances that are dissolved in it as well. Therefore the pigments are different, they are at different rates. The lines on the paper show where the pigment changes.
Pigments in Photosynthesis: Plants have chlorophyll's which has oxygen and nitrogen in it. Chlorophyll a is the main pigment in photosynthesis in plants. It's a reaction of photo systems. Carotenoids protect photosynthetic system from damaging effects of UV rays. Plants absorb everything except green in the light spectrum which is why we see green.